multisite gateway®three-fragment vector construction kit Search Results


multisite gateway entry vectors pbsdonr p1 p4  (Thermo Fisher)
99
Thermo Fisher multisite gateway entry vectors pbsdonr p1 p4
Multisite Gateway Entry Vectors Pbsdonr P1 P4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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multisite gateway t dna destination vector palligatorr43  (Thermo Fisher)
99
Thermo Fisher multisite gateway t dna destination vector palligatorr43
Multisite Gateway T Dna Destination Vector Palligatorr43, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multisite gateway cassette  (Promega)
90
Promega multisite gateway cassette
Multisite Gateway Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgem-t easy vector  (Promega)
90
Promega pgem-t easy vector
Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pgem-t easy vector - by Bioz Stars, 2026-08
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three fragment vector construction kit  (Thermo Fisher)
99
Thermo Fisher three fragment vector construction kit
Three Fragment Vector Construction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pmc03642064-272-9-13?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
three fragment vector construction kit - by Bioz Stars, 2026-08
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recombinant proteins multisite gateway three fragment vector construction kit invitrogen 12537023 catalase sigma c1345 trypsin sigma t1426  (Thermo Fisher)
99
Thermo Fisher recombinant proteins multisite gateway three fragment vector construction kit invitrogen 12537023 catalase sigma c1345 trypsin sigma t1426
Recombinant Proteins Multisite Gateway Three Fragment Vector Construction Kit Invitrogen 12537023 Catalase Sigma C1345 Trypsin Sigma T1426, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pmc07902905__mmc8-136-58-67?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
recombinant proteins multisite gateway three fragment vector construction kit invitrogen 12537023 catalase sigma c1345 trypsin sigma t1426 - by Bioz Stars, 2026-08
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multisite recombination gateway cloning system  (Addgene inc)
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Addgene inc multisite recombination gateway cloning system
Multisite Recombination Gateway Cloning System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
multisite recombination gateway cloning system - by Bioz Stars, 2026-08
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pgl3 basic vector  (Promega)
90
Promega pgl3 basic vector
Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region <t>(pGL3-basic</t> vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.
Pgl3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pmc02910055-60-1-4?v=Promega
Average 90 stars, based on 1 article reviews
pgl3 basic vector - by Bioz Stars, 2026-08
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attr1 attr2 destination vector pypq203  (Addgene inc)
93
Addgene inc attr1 attr2 destination vector pypq203
Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region <t>(pGL3-basic</t> vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.
Attr1 Attr2 Destination Vector Pypq203, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pm39950393-202-14-18?v=Addgene+inc
Average 93 stars, based on 1 article reviews
attr1 attr2 destination vector pypq203 - by Bioz Stars, 2026-08
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destination vector  (Addgene inc)
96
Addgene inc destination vector
Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region <t>(pGL3-basic</t> vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.
Destination Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pmc06064391-109-1-16?v=Addgene+inc
Average 96 stars, based on 1 article reviews
destination vector - by Bioz Stars, 2026-08
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venusyfp  (Addgene inc)
91
Addgene inc venusyfp
Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region <t>(pGL3-basic</t> vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.
Venusyfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multisite+gateway%C2%AEthree-fragment+vector+construction+kit/pm36604579-310-34-38?v=Addgene+inc
Average 91 stars, based on 1 article reviews
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Image Search Results


Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region (pGL3-basic vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.

Journal: Nucleic Acids Research

Article Title: Cell-type-specific long-range looping interactions identify distant regulatory elements of the CFTR gene

doi: 10.1093/nar/gkq175

Figure Lengend Snippet: Fine mapping of the looping interactions between the CFTR promoter and elements III and IV, and luciferase assays showing that these two elements can synergistically activate the CFTR promoter. 3C primers and data can be found in Supplementary Tables S1 and S2 . ( A–C ) 3C profiles using BsrGI to digest the chromatin focused on element III (A), anchored at the TSS. The interaction peak corresponds to a 1066 bp fragment. (B) 3C focused on element IV, anchored at the TSS. The interaction peak corresponds to a 1560 bp fragment. (C) 3C focused on element IV anchored at element III. The interaction peak corresponds to the same 1560 bp fragment that interacts with the CFTR TSS. ( D ) Outline of the fragments used in the luciferase assay. Element III was split into two fragments; element IV was split into three fragments. ( E ) Relative luciferase expression data, where ‘Prom’ refers to the fragment containing only the CFTR TSS and promoter region (pGL3-basic vector-promoter alone construct; see ‘Materials and Methods’ section), and was set as 1, and ‘intr1’ is a known enhancer element from intron 1 of the CFTR gene used as a positive control. Only element IVc showed enhanced luciferase expression compared to the promoter alone [ P <0.05 ( t -test) for both Caco2 and HepG2 cells]. ( F ) When elements IIIb and IVc were combined, a synergistic enhancer effect was observed only in the CFTR -expressing cell line Caco2 [ P <0.01 ( t -test)] when compared to element IVc alone in Caco2 cells. Asterisks indicate statistically significant differences from [Prom] (Panel E), or from [IIIb+IVc in HepG2] (Panel F). * P < 0.05; ** P < 0.01.

Article Snippet: The pGL3 basic vector (Promega) was transformed into a Gateway-compatible destination vector by inserting the R4R2 cassette into the Mlu I restriction site according to the MultiSite Gateway Cloning manual (Invitrogen).

Techniques: Luciferase, Expressing, Plasmid Preparation, Construct, Positive Control